An asexual strain of the freshwater planarian species S. mediterranea was kept in Milli-Q water containing 1.2 mM NaHCO3, 1.6 mM NaCl, 1.0 mM CaCl2, 1.0 mM MgSO4, 0.1 mM MgCl2 and 0.1 mM KCl. Planarians were continuously maintained in the dark at a temperature of 20 °C. Once a week they were fed with veal liver. Animals used in experiments were starved for at least 7 days before the procedure.

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