Normality and homogeneity of variance were analyzed using the Shapiro-Wilk and Levene tests respectively59. The differences between % of hatched eggs per female were analyzed with Mann–Whitney U tests (p = 0.05). The Kaplan–Meier survival estimator was used for generating survival functions. The differences between average of dead larvae per concentration per entomopathogenic bacteria strain were analyzed with Wilcoxon matched-pairs singed rank tests. Linear regressions were plotted to observe the differences in mortality per concentration between w+PipSJ and wPipSJ lines. For hatching rate data, an unpaired t-test comparison was used. Statistical analyses of the survival data were performed using GraphPad Prism version 4.01 for Windows (GraphPad Software, La Jolla California USA, www.graphpad.com). To establish the 24 h LC50 Log-Probit was applied, adding the 95% confidence intervals (CI) from R, specifically the package ‘ecotox’, v1.3.3, (CRAN60). Significant differences between the LC50 of the different lines were evaluated with ratio tests: if the 95% confidence interval is including 1, the difference of LC50 values is not considered significant61.

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