Mosquito larvae were collected in natural breeding sites from San Juan province, Argentina. Insects were identified based on the larval fourth stage or adult females’ morphological characters and male genitalia (those reared to adult) according to Rossi et al.42 and Harbach43 respectively. Mosquito laboratory lines were established from a naturally infected population and were maintained at the Insect Biological Control Laboratory of the INBIOTEC-CONICET, FIBA (Argentina) following lab conditions such as 24 °C and 80 ± 5% RH and a 12 h light: 12 h dark photoperiodicity. Larvae were fed using fish food (Shulet Carassius). Adult stages were provided with a 10% solution of sucrose was given to adults and females were allowed to feed blood from mice.

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