Fasting blood samples were obtained during the baseline MASALA clinical visit (2010–2013 or 2017–2018) by a trained phlebotomist. EDTA plasma was isolated and stored at − 80 °C in the MASALA core research lab. Samples were pulled and shipped to the Genomics, Proteomics, Bioinformatics and Systems Biology Center at Beth Israel Deaconess Medical Center in Boston, Massachusetts for analysis.

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