All chemicals were of analytical reagent grade. The hydrochloric acid (37%), sodium oxalate (≥ 99.5%), deuterium oxide (99%D) and sodium azide (≥ 99.0%) were purchased from Sigma-Aldrich (Milan, Italy), while sodium salt of (trimethylsilyl)-propionic-2,2,3,3-d4 acid (TSP, 99%D) was purchased from Armar Chemicals (Döttingen, Switzerland). Acetonitrile and methanol LC/MS grade and isopropanol HPLC grade were purchased from Sigma-Aldrich (Milan, Italy). Water was doubly deionised (resistivity: 18 MΩ cm) with a Milli-Q water purification system (Merck Millipore, Darmstadt, Germany).

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