During the HFD feeding period, mice were weighed regularly to record delta weight gain as the primary obesity endpoint. Additional obesity endpoints included final absolute body weight after completing HFD, overall adiposity (i.e. the total mass of all adipose tissue depots), and absolute mass of specific adipose tissue depots. Histological analysis of subcutaneous, epidydimal, and mesenteric adipose tissue depots allowed determination of the average adipocyte cell size, a determinant of white adipose tissue (WAT) quality [13].

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