The results of the expression of VP2 in the two expression strains were analyzed,VP2 protein in Rosetta (DE3) strain mainly existed in soluble form. Then, the Rosetta (DE3) strain was selected to express the target protein VP2. Moreover, the expression conditions for pET28a-VP2 (Rosseta) containing the temperature, IPTG concentration and time were explored. Recombinant protein VP2 was expressed in a series of inducting temperatures including 16 °C, 25 °C, 30 °C and 37 °C. The fusion protein was induced by a group of IPTG at varied concentrations containing 0.2 mmol/L, 0.4 mmol/L, 0.6 mmol/L, 0.8 mmol/L and 1.0 mmol/L. Afterwards, the optimal expression time was determined according to the arithmetic progression including five values increasing from the beginning value 0 h to the ending value 10 h, using the optimal temperature and IPTG concentration. All results are analyzed using SDS-PAGE.

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