After being fixed on a glass slide, the mice tumor tissue slices were soaked in citrate antigen repair solution (AR0024, Boster, Wuhan, China) and heated in a microwave oven for 8 min. Then the tissue slides were cooled naturally at room temperature and incubated with BRD4 (1:400, ab128874, Abcam) at 4 °C overnight. The next day, the tissue slides were incubated with the secondary antibody (ab205718, Abcam) for 2 h and stained with the DAB buffer for 35 min. After 2 min of washing under tap water, hematoxylin (H810910, Macklin) was added to stain the nuclei of the cells for 4 min. Finally, after the tissue slides were sealed, the images of the tissue slices were recorded using a BX53 optical microscope (Olympus, Japan).

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