After transfection, cells were suspended in basic medium to adjust the cell concentration to 500/ml. Then 2 ml of cell suspension was added into a 6-well plate. The cells were then cultured for 14 days and the basic medium was renewed every three days. Afterwards, the cell colonies were fixed with methanol (R007536, Rhawn) for 15 min, stained with crystal violet for 15 min, and washed with PBS. Finally, the cell colonies were observed and the images were documented with a camera (EOS M50, Canon, Japan).

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