After transfection, cells were suspended in complete medium to adjust the cell concentration to 4.0 × 105/ml. Then 2 ml of cell suspension was added into a 6-well plate. After the wells were filled with cells, wounds of the same width were created in each well. Then the previous medium was replaced by 2 ml of basic medium. The images of the wounds were documented at 0 and 48 h under a BX53 optical microscope (Olympus, Japan). The data were analyzed using Image J 1.8.0 software.

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