Mice femurs were scanned using a microCT instrument (Model 1172, SkyScan, Kontich, Antwerp, Belgium). The morphometric parameters selected for the analysis of bone microarchitecture are described in Table 1. All the parameters were named according to the Parfitt’s system (40).

These parameters were determined in the distal portion of the femurs, starting 0.25 mm proximal to the distal growth plate and covering a total length of 1 mm. Cortical bone was manually excluded, separating only the trabecular bone, since osteoporosis affects primarily this region of the bone in the early stages of the disease (41). The bones were scanned with an energy level of 55 kVp, tube current of 145 mA and a voxel of 5.0 µm3 (32,36). The equipment underwent a weekly programmed calibration, with phantoms presenting with densities of 0.25 and 0.75 mg/cm3. CT-analyzer software (version 1.13.2.1) was used for the quantitative assessment and CTVol (version 2.2.3.0) and Dataview (version 1.4.4.0) software were used to reconstruct images of the bones. The results are expressed according to the standard nomenclature (36).

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