Statistical Parametric Mapping (SPM)12 (London, UK) software package in Matlab v.2013b (MathWorks, Natick, MA, USA) was used to process T1 and task-based fMRI data. Task-based fMRI volumes of LtoR and RtoL motor and language task fMRI runs were realigned to the mean volume of each run. T1 volumes were coregistered with realigned task-based fMRI volumes, and all volumes were normalized to MNI space using the standard segmentation method for group-level analysis. Task-based fMRI volumes were smoothed using an isotropic Gaussian kernel with 4 mm full width at half-maximum (FWHM). A larger FWHM improves SNR but reduces spatial resolution. The voxel size of our data was 2 mm × 2 mm × 2 mm; setting the FWHM to 2 to 3 times the voxel size is considered acceptable (24,25). As high spatial resolution was critical for our analyses, we changed the FWHM of the isotropic Gaussian kernel to 4 mm in SPM12. A flow diagram of tasked-based fMRI data preprocessing is shown in Figure 1.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.