To develop a comprehensive combined transcriptome assembly, we sampled axillary shoot apical tissues prior to foliar Ag2S2O3 application (not shown in RNA-Seq study results). After 12 h light induction of flowering at day 0, foliar Ag2S2O3 application was made at days 1 and 3. Flower buds were sampled at days 8 and 14 post-flowering light induction. Samples of flower buds collected from each flower sex type at days 14 are shown in Fig. 1. The terminology refers to Ag2S2O3-induced-male flowers (IMF), normal female flowers (FF) and normal male plant (MF), used in differential gene expression studies. Axillary shoot apical tissue, and floral bud tissue samples were manually harvested and immediately flash-frozen in liquid nitrogen and stored at -80 °C until used. Frozen tissues (250 mg) of each sample were processed for total RNA purification using RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s protocol. Isolated RNA samples were then used for RNA-Seq and qPCR studies.

Floral bud samples of cannabis plants used for RNA-Seq studies between flower sex types. The flower sex types are normal male flower buds from genetically male plants; Ag2S2O3-induced male flower buds from genetically female plants; and normal female flower buds from genetically female plants. These flower buds were collected for RNA isolation at 14 days of post-light and fertigation induction of flowering

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