Cells plated in YPD medium were initially inoculated in 5 mL YPD and grown overnight (28 °C, 200 rpm on a rotary shaker). Then cells were transferred to 200 mL YPD in a 1 L shake flasks and grown overnight at the same conditions. The culture was washed twice with distilled water and diluted down to an initial optical density (OD) at 600 nm of 5 in 50 mL of medium in 250 mL shake flasks. The culture was incubated for 30 h at 28 °C and 200 rpm. Samples for transcriptome were withdrawn after 4 h of incubation and samples for metabolite analysis were withdrawn regularly. All experiments were carried out in biological triplicate.

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