Cells plated in YPD medium were initially inoculated in 5 mL YPD and grown overnight (28 °C, 200 rpm on a rotary shaker). Then cells were transferred to 200 mL YPD in a 1 L shake flasks and grown overnight at the same conditions. The culture was washed twice with distilled water and diluted down to an initial optical density (OD) at 600 nm of 5 in 50 mL of medium in 250 mL shake flasks. The culture was incubated for 30 h at 28 °C and 200 rpm. Samples for transcriptome were withdrawn after 4 h of incubation and samples for metabolite analysis were withdrawn regularly. All experiments were carried out in biological triplicate.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.