Fresh HBV-positive and HBV-negative HCC liver samples were collected from surgical resection and immediately stored in liquid nitrogen, then the tissue were cut into 5-μm-thick sections and adhered to slides. The tissue was washed with phosphate-buffered saline (PBS) and fixed in 3.7% formaldehyde for 10 min. After washing the slides in 2 × sodium citrate buffer with a solution of 10% formamide, 4 μl fluorescent probes were added to the hybridization solution, which contained 10% dextran sulfate, 10% formamide, and 2 × sodium citrate buffer. Hybridization using MINPP1 and miRNA-30b-5p probes were performed overnight at 37 °C. The slides were rinsed twice for 20 min in 2× sodium citrate buffer with a solution of 10% formamide, then counterstained with 4′-6’diamidino-2-phenylindole (DAPI). The miRNA-30b-5p was labeled with 6-carboxy-fluorescein fluorophore (CY3) while MINPP1 was labeled with cyanine dye 3 (FAM). The location of MINPP1 and miRNA-30b-5p was detected using confocal laser scanning microscopy (Leica, Wetzlar, Germany).

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