RNA isolation and cDNA synthesis

RNA isolation was performed, with some modification, according to the guanidium-acid-phenol extraction protocol described by Farrell (2010). DNase application was performed as recommended by the manufacturer (DNase I recombinant-Sigma-Aldrich, Germany). For cDNA synthesis, Roche Transcriptor High Fidelity cDNA synthesis kit (Sigma-Aldrich, Germany) protocol was applied. At the end of the process, samples were stored at − 20 °C until use in the study.

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