Effect of TA on H1N1-Infection In Vitro
This protocol is extracted from research article:
Anti-microbial Effects In Vitro and In Vivo of Alstonia scholaris
Nat Prod Bioprospect, Jan 3, 2021; DOI: 10.1007/s13659-020-00294-6

The efficacy of TA against H1N1 virus in vitro was studied by cultivation of chicken embryo and haemagglutination inhibition test as previously reported [62]. The proliferative activity of influenza virus H1N1 was determined by chicken embryo method and the virus titers was 1/1280. All embryonated eggs except the normal group were injected with 0.1 mL H1N1 virus (60 U/mL) via allantoic cavity inoculation before treatment. Then the eggs inoculated virus were randomly divided into 5 groups. The embryonated eggs in model group received 0.1 mL of 1% DMSO, the embryonated eggs in the positive group received 0.1 mL of control; group 3 to 5 were administrated different concentrations of TA treatment, and finally, the normal group was also inoculated with 0.1 mL of 1% DMSO. The concentration of TA was non-toxic to chicken embryos confirmed by a preliminary experiment (data not shown). Allantoic fluid of chicken embryo were collected from each group at 48 h post infection, and the hemagglutination titer (HI) was determined by hemagglutination assay with 1% chicken red blood cell. The virus titer of H1N1 declined by one dilution was considered to be effective, and significant effect by two dilutions, when compared the model group.

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