Anti- HSV-1 and—RSV Activity Assay In Vitro
This protocol is extracted from research article:
Anti-microbial Effects In Vitro and In Vivo of Alstonia scholaris
Nat Prod Bioprospect, Jan 3, 2021; DOI: 10.1007/s13659-020-00294-6

Methods was referred to the previous with minor modifications [60], TA was suspended in 1% dimethylsulfoxide/sterile water for injection. Equivalent of 100 folds TCID50/mL of RSV for Hep2 cells and 10 folds TCID50/mL of HSV-1 for Vero cells were added to the cell cultures which were then incubated at 37 °C in a 5% CO2 atmosphere, and the supernatant was removed after incubation for 2 h. Cells were then treated with serial concentrations of TA diluted from TC50 to 1/64 TC50 (mg/mL) or with two-fold serial dilutions of ribavirin and acyclovir (from 1/2 TC50 to 1/128 TC50) used as the positive control. All wells were then observed under a light microscope to determine the virus-induced cytopathic effect (CPE) at day 5 after the treatment. The concentrations of the test articles that reduced CPE by 50% (IC50) were determined. And the therapeutic index (TI) value was calculated as the ratio TC50/IC50 [61].

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.