Chemicals, Viruses, Bacteria and Cells
This protocol is extracted from research article:
Anti-microbial Effects In Vitro and In Vivo of Alstonia scholaris
Nat Prod Bioprospect, Jan 3, 2021; DOI: 10.1007/s13659-020-00294-6

Acyclovir and ribavirin were selected to be antiviral control against HSV-1 and RSV and provided by Tianjin Pharmaceutical Jiaozuo Co, Ltd (Tianjin, China). Kugan granule, a launched traditional Chinese medicine formula, was used to be antiviral control against H1N1, and provided by Qingdao Guofeng Pharmaceutical Co. Ltd (Qingdao, Shandong). Amoxicillin was chosen as an antibacterial control and produced by Zhuhai Federal Pharmaceutical Co. Ltd (Hongkong, China).

The influenza strains A/FM1/1/47 (H1N1) and herpes simplex virus type 1 strain sm44 (HSV-1) were obtained from National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention. Respiratory Syncytial Virus (RSV) was provided by West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University. Hep2 and Vero cells were purchased from Conservation Genetics CAS Shanghai Cell Bank. Specific-pathogen-free embryonated eggs were denoted by West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University. Standard strain beta-hemolytic streptococcus was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

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