The bacterial strains for this experiment were isolated from the wounds of lymphoedema patients from East Wollega Zone. Standard reference bacteria were obtained from the National Referral Bacteriology and Mycology Laboratory, Ethiopian Public Health Institute. Clinical isolates of Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Shewanella alage were used. Reference bacteria including MRSA Staphylococcus aureus ATCC®43300™, Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC700603 and Pseudomonas aeruginosa ATCC37853 were also used.

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