96-well non-tissue culture treated plate wells were coated with 1 μg Fc variant protein per well overnight at 4 °C, and the wells were washed once with PBS and added with 1×105 Jurkat-NFAT-FcγRIIIa reporter cell line. After 24 h of incubation in CO2 incubator at 37 °C, the luciferase activity of cells was detected using Luciferase Assay System (Promega Corporation).

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