HEK293T cells were cultured in DMEM medium (Thermo Fisher Scientific). Jurkat-NFAT-FcγRIIIa reporter cell line, CD40 reporter cell line and Ramos cells were cultured in RPMI 1640 medium (Thermo Fisher Scientific). SK-BR-3 cells were cultured in McCoy's 5A (Modified) medium (Biological Industries). All the culture medium was supplemented with 10% fetal bovine serum (Biological Industries), 1× non-essential amino acids, 100 U/mL penicillin, 100 μg/mL streptomycin and 12.5 mM HEPES (Thermo Fisher Scientific). HEK293F cells were suspension cultured in FreeStyle™ 293 Expression Medium (Thermo Fisher Scientific). CHO-K1 or FUT8-/- CHO-K1 cells were cultured in FreeStyle™ CHO Expression Medium (Thermo Fisher Scientific). All the cells were maintained in CO2 incubator at 37 °C.

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