For co-IP, the plasmids expressing Flag-AIB1 or MafB were cotransfected into 293T cells, and the cells were lysed 24 h after transfection. The cell lysates were immunoprecipitated with the corresponding antibodies or control IgG. After washing, the precipitates were analyzed by western blotting. For the GST pull-down assay, the GST-MafB proteins or GST were incubated with glutathione Sepharose 4B beads (GE Healthcare), followed by the addition of the lysates of cells overexpressing Flag-AIB1 or the Flag-AD2 domain. After incubation, the beads were washed and analyzed by western blotting.

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