SKOV3 and A2780 cells were seeded in 24-well plates (1.5 × 103 cells/well) in triplicate. After 24 h of culture, the cells were cotransfected with the desired firefly luciferase reporter plasmid (400 ng/mL, Genepharma) and TK-Renilla luciferase plasmid (20 ng/mL, Genepharma) using Lipofectamine 2000 reagent. Luciferase and Renilla signals were determined 24 h after transfection using a Dual Luciferase Reporter Assay Kit (GN201-01, YPH-bio, Co. Ltd. Beijing, China) on a GloMax 20/20 luminometer (Promega, Madison, WI, USA).

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