H22 and 4T1 cells were seeded in a 96-well plate at a density of 1×104 cells/well under normoxia (21% O2) or hypoxia (2% O2) overnight. The cells were then treated with Mn-TCPP, MOF or Mn-MOF at different Zr concentrations in the presence or absence of 10 nM UAMC-3203 for 10 h under normoxia or hypoxia at 37 °C. The cells were washed with PBS and then irradiated with or without US (1 MHz, 0.9 W/cm2, 30% duty cycle) for 10 min. The cells were further incubated for 24 h and the cell viability was determined using MTT assay. Briefly, the cells were washed with PBS and then incubated in the media containing 0.5 mg/mL MTT at 37 °C for another 4 h. The media were discarded and 150 μL DMSO was added to dissolve formazan crystals. The absorbance of formazan at 490 nm was measured by a Labsystems iEMS microplate reader (Helsinki, Finland).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.