H22 and 4T1 cells were seeded in a 96-well plate at a density of 1×104 cells/well under normoxia (21% O2) or hypoxia (2% O2) overnight. The cells were then treated with Mn-TCPP, MOF or Mn-MOF at different Zr concentrations in the presence or absence of 10 nM UAMC-3203 for 10 h under normoxia or hypoxia at 37 °C. The cells were washed with PBS and then irradiated with or without US (1 MHz, 0.9 W/cm2, 30% duty cycle) for 10 min. The cells were further incubated for 24 h and the cell viability was determined using MTT assay. Briefly, the cells were washed with PBS and then incubated in the media containing 0.5 mg/mL MTT at 37 °C for another 4 h. The media were discarded and 150 μL DMSO was added to dissolve formazan crystals. The absorbance of formazan at 490 nm was measured by a Labsystems iEMS microplate reader (Helsinki, Finland).

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