FVB mice were treated with vehicle, LL6 (20, 40, and 80 mg/kg, dissolved in 10% DMSO in 60% PEG 400 solution) or combination of linsitinib (25 mg/kg, dissolved in a 25 mM tartaric acid solution) and dasatinib (20 mg/kg, dissolved in an 80 mM citric acid solution) daily for 12 days. Blood was collected from euthanized mice under isoflurane-induced deep anesthesia by cardiac puncture. After allowing blood coagulation at 4 °C, serum was collected by centrifugation at 3,000 rpm for 10 min at 4 °C. Analysis of the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) was performed using a veterinary hematology analyzer (Fuji DRI-Chem 3500s, Fujifilm, Tokyo, Japan) according to the manufacturer' s protocols.

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