H22 and 4T1 cells were seeded in a 12-well plate at a density of 1×105 cells/well overnight. The cells were then treated with Mn-TCPP, MOF or Mn-MOF at Zr concentration of 10 μg/mL and Mn concentration of 2 μg/mL for different time intervals. The cells were washed with PBS three times and then the intracellular accumulation of TCPP was determined by flow cytometry and confocal microscope. For confocal microscopic analysis, the cells were stained with 2.5 μg/mL DAPI for 15 min and 50 nM LysoTracker green for 45 min at 37 °C and then observed by an Olympus FV1000 confocal microscope (Tokyo, Japan).

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