Kd values were obtained by KINOMEscan from DiscoverX (San Diego, CA, USA). To briefly summarize, Kd was measured by a competition binding assay that quantitatively measures the ability of a compound to compete with an immobilized ligand. The ligand-displayed affinity bead and DNA (T7 bacteriophage)-tagged kinases are combined with a test compound, and the amount of fusion protein bound to the solid support is quantified by qPCR. Dose response curves of 11 points are obtained for Kd values, which are measured in duplicate. See more details in the cited references 39-42 and see also at www.discoverx.com.

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