Kd values were obtained by KINOMEscan from DiscoverX (San Diego, CA, USA). To briefly summarize, Kd was measured by a competition binding assay that quantitatively measures the ability of a compound to compete with an immobilized ligand. The ligand-displayed affinity bead and DNA (T7 bacteriophage)-tagged kinases are combined with a test compound, and the amount of fusion protein bound to the solid support is quantified by qPCR. Dose response curves of 11 points are obtained for Kd values, which are measured in duplicate. See more details in the cited references 39-42 and see also at www.discoverx.com.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.