Anti-TGFBI antibodies were radiolabeled using a previously described three-step procedure 24: (1) antibody coupling of the p-isothiocyanatobenzyl-desferrioxamine chelate, (2) chelated antibody radiolabeling with 89Zr oxalic acid, and (3) radiolabeled antibody purification by exclusion chromatography on a Sephadex G25 matrix. After radiolabeling yield and volume activity evaluation, thin layer chromatography was performed to check the absence of free 89Zr in the radiolabeled antibody solution. Finally, the radiolabeled TGFBI antibody antigen-binding activity was evaluated by ELISA.

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