Anti-TGFBI antibodies were radiolabeled using a previously described three-step procedure 24: (1) antibody coupling of the p-isothiocyanatobenzyl-desferrioxamine chelate, (2) chelated antibody radiolabeling with 89Zr oxalic acid, and (3) radiolabeled antibody purification by exclusion chromatography on a Sephadex G25 matrix. After radiolabeling yield and volume activity evaluation, thin layer chromatography was performed to check the absence of free 89Zr in the radiolabeled antibody solution. Finally, the radiolabeled TGFBI antibody antigen-binding activity was evaluated by ELISA.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.