Cells were seeded into 96-well plates at a density of 1-2 × 103 cells/well and incubated for 24 h. Cells were treated with vehicle or various concentrations of test compounds for two (PC9 and PC9/ER cells) or three (other cells) days. Cells were further incubated with MTT solution (final concentration of 500 μg/mL) for 4 h at 37 °C. The formazan products were dissolved in dimethyl sulfoxide (DMSO), and the absorbance was measured at 570 nm. The data are presented as a percentage of the control group.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.