The neutral comet assay was performed using Comet assay kits (Trevigen, Maryland, USA) according to the manufacturer's instructions. SYBR green I nucleic acid stain (Biosharp, Wuhan, China) was used to stain DNA. Images were taken on an epifluorescence microscope (Olympus IX71, Olympus, Japan) at × 10 magnification. Tail moments were analyzed using OpenComet software ( 25.

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