Cells were seeded in 96-well plates and treated with the indicated agents. CCK-8 (Dojindo, Kumamoto, Japan) solution was added to each well, and the plates were incubated at 37 °C for 2 h. The absorbance at 450 nm was then measured using a microplate reader (BioTek Instruments Inc, USA). The viability of the cells in the experimental groups was normalized to that of the cells in the control group.

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