1×106 PDTALL13 cells were IV injected in 32 irradiated NRG mice. Mice partial body irradiations were performed using a rotational X-ray irradiator (Xenx, Xstrahl, Experimental Radiotherapy Core Facility, IRCM, Montpellier, France). Single doses of 4.5 Gy were delivered at a dose rate of 2.7 Gy/min (225 kV and 13.6 mA) to the mice under sedation. After 1 week, mice were randomized (n = 8 mice/group) and treated with vehicle or anti-IgG control, 20 mg/kg/week of demcizumab (anti-human DLL4 antibody, Oncomed, Redwood City, CA, USA), 20 mg/kg/week of anti-mouse DLL4 antibody (Oncomed), or 3.3 mg/kg/day, from Monday to Thursday, of dibenzazepine (DBZ) (Synkon, Groningen, Netherlands), as previously described 26.

Spleens of moribund mice treated as above were formalin fixed and paraffin-embedded. For Immunofluorescence, the following antibodies were used: anti-HES1 (clone D6P2U, # 11988, Cell Signaling Technology); anti-human vimentine (vimentine (h); clone V9, # 790-2917), anti-mouse/human vimentine (vimentine (m/h); clone D21H3, #7541, Cell Signaling Technology) and DAPI (Sigma). All stainings were performed using Discovery Ultra automated system (Ventana) at RHEM (Réseau d'Histologie Expérimentale de Montpellier). Images were acquired using Thunder Imaging System (Leica) microscope. For each spleen, 4 random fields (×20 magnification) were analyzed using ImageJ software.

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