Cell lysates were separated on 10% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE). Western blotting was performed as described in our previous study 31. The antibodies used were against human proteins ITGB3 (#4702,CST,USA), integrin alpha V beta 3 (ab78289, Abcam, Cambridge, UK), CDC42 (#2466,CST, NJ, USA), F-actin (#206,Abcam, Cambridge, UK) , NUPR1 (#6028, Abcam, Cambridge, UK) , YAP1 (#52771, Abcam, Cambridge, UK) , and p-YAP1-S127 (#76252, Abcam, Cambridge, UK). β-actin (#179467, Abcam, Cambridge, UK) was used as the protein loading control.

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