Cell lysates were separated on 10% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE). Western blotting was performed as described in our previous study 31. The antibodies used were against human proteins ITGB3 (#4702,CST,USA), integrin alpha V beta 3 (ab78289, Abcam, Cambridge, UK), CDC42 (#2466,CST, NJ, USA), F-actin (#206,Abcam, Cambridge, UK) , NUPR1 (#6028, Abcam, Cambridge, UK) , YAP1 (#52771, Abcam, Cambridge, UK) , and p-YAP1-S127 (#76252, Abcam, Cambridge, UK). β-actin (#179467, Abcam, Cambridge, UK) was used as the protein loading control.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.