The TdT-mediated dUTP nick-end labeling (TUNEL) in situ cell death detection kit Fluorescein (Roche Diagnostics, Mannheim, Germany) was used to determine cell apoptosis. In brief, the slides were deparaffinized and rehydrated, then incubated with TUNEL reaction mixture for 1 h, followed by DAPI staining. The images were taken, and the percentage of apoptotic cells was counted.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.