Copy number analysis was performed following the protocol reported previously with minor modifications 91. Briefly, MCF7 cells cultured in stripping medium for three days were treated with estrogen (10-7 M) for 6 h, and 1.5 × 106 cells were then used for copy number analysis. Absolute quantification was performed using 2-fold serial dilutions of the reference standard, and Ct value from qPCR analysis versus the dilution factor was plotted, fitting the data to a straight line. The standard curve was used for extrapolating the number of molecules of interest.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.