SiRNA-transfected cells were re-seeded at confluence in 6-well plates, and wounds were performed with a P200 pipette tip. After removing cellular debris by washing cells with PBS, three images of each well were taken. The wounded area was measured by using image J and recorded as A0. The cells were then allowed to migrate into the wounded area, and three images were taken and the wounded area was measured again 36 or 48 h later and recorded as A1. Cell migration was presented as wound closure (%) = (wounded area (A0-A1)/wounded area A0) × 100%.

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