Chromatin isolation by RNA purification (ChIRP)
This protocol is extracted from research article:
Functional roles of antisense enhancer RNA for promoting prostate cancer progression
Theranostics, Jan 1, 2021; DOI: 10.7150/thno.51931

The ChIRP experiment was performed essentially following the original protocol as described previously 15. The online Biosearch Technologies' Stellaris FISH Probe Designer was used to design antisense oligo probes tiling PSA and FKBP5 antisense eRNA. The probe oligos were synthesized with a 3'-Biotin-TEG modification and purified by HPLC. The ChIRP probes are shown in Table S1.

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