To construct ceRNA network, miRNAs that could bind to circPGR were first predicted by using four independent algorithms, miRanda (default settings) 73, RNAhybrid (minimal free energy, -e -23) 74, TarPmiR (probability of target site, -p 0.8) 75 and RegRNA2 72, based on miRBase. The miRNAs that were commonly predicted, miR-301a-5p, miR-612 and miR-3619-3p, were chosen for downstream analysis. mRNA targets that these three miRNAs could target to were predicted with Targetscan 86. To construct the ceRNA network for circPGR, only the mRNA targets that were shown to be regulated by circPGR were kept. The ceRNA network was constructed by Cytoscape 87.

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