TC664 and TC712 after different treatments were seeded in six-well plates at a density of 400 cells/well. Cells were treated with 200 μM TMZ every three days. Two weeks later, cell colonies were fixed and stained with crystal violet. We defined a colony as 50 cells.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.