Total RNA was isolated using Trizol (Invitrogen) following the manufacturer's protocol. First-strand cDNA synthesis from total RNA was carried out using GoScript™ Reverse Transcription Mix (Promega, random primers), followed by quantitative PCR (qPCR) using AriaMx Real-Time PCR machine (Agilent Technologies). RNA samples from three biological repeats were pooled together for RT-qPCR analysis, and at least three technical repeats have been done for each pooled sample. Standard error of the mean is depicted. Sequence information for all primers used to check both gene expression and circPGR expression were presented in Supplementary Table 3 (Table S3).

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