Total, cytoplasmic, and nuclear RNA were extracted using TRIzol reagent according to the manufacturer's instructions 23, 24. EV-RNA extraction was performed, as previously described 24. A stem-loop-specific primer method was used to measure miR-30b-p expression, as described previously 25. The cDNA was amplified by qRT-PCR using SYBR® Premix Ex Taq™ (Takara, Kusatsu, Japan) on a 7900HT system (Applied Biosystems/Thermo Fisher Scientific, Foster City, CA, USA). Fold-changes were calculated by relative quantification (2-ΔΔCt). The primers used for PCR are presented in Supplementary Table 3.

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