Peptides were radiolabeled with 111InCl3 (Curium) in 0.5 M 2-(N-morpholino)ethanesulfonic acid (MES) buffer (twice volume of 111InCl3), pH 5.5, for 10-30 min at 45 °C under metal-free conditions 24. Following incubation, 50 mM ethylenediaminetetraacetic acid (EDTA) was added to a final concentration of 5 mM to chelate unincorporated 111InCl3. Labeling efficiency was determined by instant thin-layer chromatography (ITLC) using silica gel-coated paper (Agilent Technologies) and 0.1 M ammonium acetate containing 0.1 M EDTA, pH 5.5, as the mobile phase. Moreover, radiochemical purity was checked using reverse-phase high performance liquid chromatography (RP-HPLC) on an Agilent 1200 system (Agilent Technologies) with an in-line radiodetector. A C18 column (5 µm, 4.6 × 250 mm; HiChrom) was used at a flow rate of 1 ml/min with the following buffer system: buffer A, triethylammonium acetate (10 mM, pH 7); buffer B, 100% methanol; and a gradient of 97% to 0% buffer A (35 min). Peptides were purified by a Sep-Pak C18 light cartridge (Waters) and eluted from the cartridge with 50% ethanol in water.

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