Cell lines were purchased from the American Type Culture Collection. LS174T colon carcinoma cells were stably transfected with human PSMA using the plasmid pcDNA3.1-hPSMA and cultured in RPMI 1640 medium supplemented with 2 mM glutamine, 10% FCS, and 0.3 mg/ml G418 at 37 °C in a humidified atmosphere with 5% CO2 5.

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