PI/Calcein-AM double stain kit (Shanghai, China) was used to detect cell apoptosis. After the required treatment, RGCs were incubated with Calcein-AM (10 μM, C3099, Invitrogen) and PI (10 μM, P3566, Molecular Probes) at 37 °C for 20 min. The images were captured using a fluorescence microscope (Olympus IX73, Tokyo, Japan). The live cells were observed at 490 nm excitation filter, while the dead cells were observed at 545 nm excitation filter.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.