OGD/R model was established to mimic ischemic injury in vitro. After the required treatment, RGCs were cultured in DMEM medium with the deoxygenated glucose-free Hanks' Balanced Salt Solution (Invitrogen). After 60 min of incubation, these cells were transferred to the hypoxic chamber with mixed gas (0.2% O2, 94.8% N2, and 5% CO2) at 37 °C. After 2 h, the cultures were subjected to reoxytenation by exchanging the medium to glucose restoration and maintained under normoxic conditions for 24 h, respectively. As the control group, these cells were grown in the full media containing glucose under normal conditions.

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