Tumor mass from mice was collected and frozen for cryostat section. After permeabilization and blocking, cryostat sections were incubated with the primary antibodies for PD-L1 (Abcam), CD8+ CTL (Cell Signaling Technology), GzyB (Cell Signaling Technology), active caspase 3 (Cell Signaling Technology) and TUNEL (Roche) overnight at 4 °C. After being washed with PBS for three times, these cryostat sections were incubated with corresponding secondary antibodies at room temperature for 1 hour. For nuclear staining, sections were incubated with DAPI. Then CLSM was used for photo imaging.

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