36 female Balb/C mice bearing 4T1 tumors (about 100 mm3) were randomly divided into 6 groups (n=6) and received different treatments including saline, anti-PD-L1, Met, SN38, Met/SN38 or MS NPs. A total of 6 injections were conducted. The tumor volume was measured every 2 days. The first administration was recorded as 0 day. A mouse was defined as dead when its tumor volume reached 1000 mm3. And survival rate is equal to the number of surviving ones divided by the total number of mice for each group.

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