4T1 cells (2×106) were inoculated subcutaneously into right legs of female Balb/C mice. When tumor volume reached ~100 mm3, female Balb/C mice were randomly divided into 6 groups (n=6). The first group of mice was injected with saline, as saline group; the second group was injected with anti-PD-L1 (2.0 mg/kg), as anti-PD-L1 group; the third group was injected with Met (11 mg/kg), as Met group; the forth group was injected with SN38 (1.1 mg/kg), as SN38 group; the fifth group was injected with Met/SN38, as Met/SN38 group (11 mg/kg Met and 1.1 mg/kg SN38); the sixth group was injected with MS NPs (12 mg/kg), as MS NPs group. Anti-PD-L1 was administered through intraperitoneal injection, other formulations were intravenously injected via tail vein once every other day for 12 days. The first administration was recorded as 0 day. The tumor volume was measured with a caliper every 2 days for 16 days, and relative tumor volume (V/V0) was calculated as the result. Body weight was measured every 2 days for 16 days. After the last measurement, mice were euthanized, tumor and major organs were collected for H&E staining.

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