Two-photon fluorescence microscopy image stacks were analyzed with Imaris (Bitplane, Belfast, UK). 3D reconstructions of blood vessels were created using the semi-automated Surface module. In images with high background noise, such as post-treatment images in which the intensity of dextran in the extravascular space was higher than that in the intravascular space, blood vessels were manually contoured. EGFP+ cells were detected using the semi-automated Surface module for XYZT images and Spots module for XYT images. Cell activity was automatically tracked using the Track module and manually corrected. For cell displacement measurements, a minimum of ten cells were tracked. Displacement was calculated as the distance between a tracked cells' first and last position.

To evaluate the proportion of blood vessels covered by fluorescent cells, the volume or area of blood vessels and cells were measured by creating a Surface in their respective channels.

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